HT-29 cell
인간 유래 대장암 세포이다. 배양시 성장 속도는 준수한 편..
배양은 DMEM 10% FBS 1% P/S 을 사용한다.
개인적으로 천연물을 이용한 추출물로 항암 효과 진단시, 효과를 보기 가장 어려운 cell 중에 하나인 것 같다.
<아래 자료는 한국세포주은행>
| Items | Contents |
| KCLB No. | 30038 |
| Name | HT-29 |
| Distributibility | Yes |
| Cell Line STR Profile |
D3S1358: 15,17 vWA: 17,19 FGA: 20,22 Amelogenin: X TH01: 6,9 TPOX: 8,9 CSF1P0: 11,12 D5S818: 11,12 D13S317: 11,12 D7S820: 10 |
| Origin | colon |
| Species | human - female, 44 years old, Caucasian |
| Receptor | urokinase receptor (u-PAR); vitamin D (moderate expression) |
| Virus Susceptibility | human immunodeficiency virus (HIV,LAV) |
| Tumorigenecity | Yes, in nude mice forms well differentiated adenocarcinoma consistent with colonic primary(grade I); tumors also form in steroid treated hamsters. |
| Isoenzyme | Me-2,1;PGM3,1-2;PGM1,1-2;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B |
| Cellular morphology | epithelial |
| Production | Secretory component of IgA; carcinoembryonic antigen(CEA); transforming growth factor beta binding protein; mucin |
| Growth pattern | monolayer |
| Histopathology | adenocarcinoma |
| Freezing media | RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5% |
| Original media | McCoy's 5a medium with 1.5mM L-glutamine, 90%; heat inactivated fetal bovine serum (FBS), 10% |
| KCLB media | RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10% |
| Subculture | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| References | Fogh J., ed., Human tumor cells in vitro. New York: Plenum Press; 1975: pp.115-159 Chen TR et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet.Cytogenet. 27: 125-134, 1987 |
| p53 mutation | codon 273 Arg->His |
| Split ratio | 1:3 to 1:8 is recommended |
| Media change | 2 to 3 times weekly |
| Oncogene | myc +; ras +; myb +; fos +; sis +;p 53 +; abl -;ros -;src - |
| Photos:Culture Morphology, STR Profiles |